NMR Spectroscopic Assignment of Backbone and Side-Chain Protons in Fully Protonated Proteins: Microcrystals, Sedimented Assemblies, and Amyloid Fibrils.
Identifieur interne : 001A71 ( Main/Exploration ); précédent : 001A70; suivant : 001A72NMR Spectroscopic Assignment of Backbone and Side-Chain Protons in Fully Protonated Proteins: Microcrystals, Sedimented Assemblies, and Amyloid Fibrils.
Auteurs : Jan Stanek [France] ; Loren B. Andreas [France] ; Kristaps Jaudzems [France] ; Diane Cala [France] ; Daniela Lalli [France] ; Andrea Bertarello [France] ; Tobias Schubeis [France] ; Inara Akopjana [Lettonie] ; Svetlana Kotelovica [Lettonie] ; Kaspars Tars [Lettonie] ; Andrea Pica [Italie] ; Serena Leone [Italie] ; Delia Picone [Italie] ; Zhi-Qiang Xu [Australie] ; Nicholas E. Dixon [Australie] ; Denis Martinez [France] ; Mélanie Berbon [France] ; Nadia El Mammeri [France] ; Abdelmajid Noubhani [France] ; Sven Saupe [France] ; Birgit Habenstein [France] ; Antoine Loquet [France] ; Guido Pintacuda [France]Source :
- Angewandte Chemie (International ed. in English) [ 1521-3773 ] ; 2016.
Abstract
We demonstrate sensitive detection of alpha protons of fully protonated proteins by solid-state NMR spectroscopy with 100-111 kHz magic-angle spinning (MAS). The excellent resolution in the Cα-Hα plane is demonstrated for 5 proteins, including microcrystals, a sedimented complex, a capsid and amyloid fibrils. A set of 3D spectra based on a Cα-Hα detection block was developed and applied for the sequence-specific backbone and aliphatic side-chain resonance assignment using only 500 μg of sample. These developments accelerate structural studies of biomolecular assemblies available in submilligram quantities without the need of protein deuteration.
DOI: 10.1002/anie.201607084
PubMed: 27865050
Affiliations:
- Australie, France, Italie, Lettonie
- Aquitaine, Auvergne-Rhône-Alpes, Nouvelle-Aquitaine, Rhône-Alpes
- Bordeaux, Villeurbanne
- Université de Bordeaux
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Dixon</name><affiliation wicri:level="1"><nlm:affiliation>School of Chemistry, University of Wollongong, NSW, 2522, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>School of Chemistry, University of Wollongong, NSW, 2522</wicri:regionArea><wicri:noRegion>2522</wicri:noRegion></affiliation></author><author><name sortKey="Martinez, Denis" sort="Martinez, Denis" uniqKey="Martinez D" first="Denis" last="Martinez">Denis Martinez</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Chemistry & Biology of Membranes & Nanoobjects (UMR 5248 CBMN - CNRS, University of Bordeaux, Bordeaux INP), All. Geoffroy Saint-Hillaire, 33600, Pessac, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Institute of Chemistry & Biology of Membranes & Nanoobjects (UMR 5248 CBMN - CNRS, University of Bordeaux, Bordeaux INP), All. Geoffroy Saint-Hillaire, 33600, Pessac</wicri:regionArea><wicri:noRegion>33600, Pessac</wicri:noRegion><wicri:noRegion>33600, Pessac</wicri:noRegion></affiliation></author><author><name sortKey="Berbon, Melanie" sort="Berbon, Melanie" uniqKey="Berbon M" first="Mélanie" last="Berbon">Mélanie Berbon</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Chemistry & Biology of Membranes & Nanoobjects (UMR 5248 CBMN - CNRS, University of Bordeaux, Bordeaux INP), All. Geoffroy Saint-Hillaire, 33600, Pessac, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Institute of Chemistry & Biology of Membranes & Nanoobjects (UMR 5248 CBMN - CNRS, University of Bordeaux, Bordeaux INP), All. 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Geoffroy Saint-Hillaire, 33600, Pessac</wicri:regionArea><wicri:noRegion>33600, Pessac</wicri:noRegion><wicri:noRegion>33600, Pessac</wicri:noRegion></affiliation></author><author><name sortKey="Noubhani, Abdelmajid" sort="Noubhani, Abdelmajid" uniqKey="Noubhani A" first="Abdelmajid" last="Noubhani">Abdelmajid Noubhani</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Chemistry & Biology of Membranes & Nanoobjects (UMR 5248 CBMN - CNRS, University of Bordeaux, Bordeaux INP), All. Geoffroy Saint-Hillaire, 33600, Pessac, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Institute of Chemistry & Biology of Membranes & Nanoobjects (UMR 5248 CBMN - CNRS, University of Bordeaux, Bordeaux INP), All. Geoffroy Saint-Hillaire, 33600, Pessac</wicri:regionArea><wicri:noRegion>33600, Pessac</wicri:noRegion><wicri:noRegion>33600, Pessac</wicri:noRegion></affiliation></author><author><name sortKey="Saupe, Sven" sort="Saupe, Sven" uniqKey="Saupe S" first="Sven" last="Saupe">Sven Saupe</name><affiliation wicri:level="4"><nlm:affiliation>Institut de Biochimie et de Génétique Cellulaire (UMR 5095, CNRS -, Université de Bordeaux), 33077, Bordeaux, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Institut de Biochimie et de Génétique Cellulaire (UMR 5095, CNRS -, Université de Bordeaux), 33077, Bordeaux</wicri:regionArea><wicri:noRegion>33077, Bordeaux</wicri:noRegion><orgName type="university">Université de Bordeaux</orgName><placeName><settlement type="city">Bordeaux</settlement><region type="region" nuts="2">Nouvelle-Aquitaine</region><region type="old region" nuts="2">Aquitaine</region></placeName></affiliation></author><author><name sortKey="Habenstein, Birgit" sort="Habenstein, Birgit" uniqKey="Habenstein B" first="Birgit" last="Habenstein">Birgit Habenstein</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Chemistry & Biology of Membranes & Nanoobjects (UMR 5248 CBMN - CNRS, University of Bordeaux, Bordeaux INP), All. Geoffroy Saint-Hillaire, 33600, Pessac, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Institute of Chemistry & Biology of Membranes & Nanoobjects (UMR 5248 CBMN - CNRS, University of Bordeaux, Bordeaux INP), All. Geoffroy Saint-Hillaire, 33600, Pessac</wicri:regionArea><wicri:noRegion>33600, Pessac</wicri:noRegion><wicri:noRegion>33600, Pessac</wicri:noRegion></affiliation></author><author><name sortKey="Loquet, Antoine" sort="Loquet, Antoine" uniqKey="Loquet A" first="Antoine" last="Loquet">Antoine Loquet</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Chemistry & Biology of Membranes & Nanoobjects (UMR 5248 CBMN - CNRS, University of Bordeaux, Bordeaux INP), All. Geoffroy Saint-Hillaire, 33600, Pessac, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Institute of Chemistry & Biology of Membranes & Nanoobjects (UMR 5248 CBMN - CNRS, University of Bordeaux, Bordeaux INP), All. Geoffroy Saint-Hillaire, 33600, Pessac</wicri:regionArea><wicri:noRegion>33600, Pessac</wicri:noRegion><wicri:noRegion>33600, Pessac</wicri:noRegion></affiliation></author><author><name sortKey="Pintacuda, Guido" sort="Pintacuda, Guido" uniqKey="Pintacuda G" first="Guido" last="Pintacuda">Guido Pintacuda</name><affiliation wicri:level="1"><nlm:affiliation>Centre de RMN à Très Hauts Champs, Institut des Sciences Analytiques (UMR 5280 - CNRS, ENS Lyon, UCB Lyon 1), Université de Lyon, 5 rue de la Doua, 69100, Villeurbanne, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Centre de RMN à Très Hauts Champs, Institut des Sciences Analytiques (UMR 5280 - CNRS, ENS Lyon, UCB Lyon 1), Université de Lyon, 5 rue de la Doua, 69100, Villeurbanne</wicri:regionArea><wicri:noRegion>69100, Villeurbanne</wicri:noRegion><wicri:noRegion>69100, Villeurbanne</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:27865050</idno><idno type="pmid">27865050</idno><idno type="doi">10.1002/anie.201607084</idno><idno type="wicri:Area/PubMed/Corpus">001500</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001500</idno><idno type="wicri:Area/PubMed/Curation">001478</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001478</idno><idno type="wicri:Area/PubMed/Checkpoint">001478</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001478</idno><idno type="wicri:Area/Ncbi/Merge">003F07</idno><idno type="wicri:Area/Ncbi/Curation">003F07</idno><idno type="wicri:Area/Ncbi/Checkpoint">003F07</idno><idno type="wicri:Area/Main/Merge">001A67</idno><idno type="wicri:Area/Main/Curation">001A71</idno><idno type="wicri:Area/Main/Exploration">001A71</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">NMR Spectroscopic Assignment of Backbone and Side-Chain Protons in Fully Protonated Proteins: Microcrystals, Sedimented Assemblies, and Amyloid Fibrils.</title><author><name sortKey="Stanek, Jan" sort="Stanek, Jan" uniqKey="Stanek J" first="Jan" last="Stanek">Jan Stanek</name><affiliation wicri:level="3"><nlm:affiliation>Centre de RMN à Très Hauts Champs, Institut des Sciences Analytiques (UMR 5280 - CNRS, ENS Lyon, UCB Lyon 1), Université de Lyon, 5 rue de la Doua, 69100, Villeurbanne, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Centre de RMN à Très Hauts Champs, Institut des Sciences Analytiques (UMR 5280 - CNRS, ENS Lyon, UCB Lyon 1), Université de Lyon, 5 rue de la Doua, 69100, Villeurbanne</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Villeurbanne</settlement></placeName></affiliation></author><author><name sortKey="Andreas, Loren B" sort="Andreas, Loren B" uniqKey="Andreas L" first="Loren B" last="Andreas">Loren B. Andreas</name><affiliation wicri:level="3"><nlm:affiliation>Centre de RMN à Très Hauts Champs, Institut des Sciences Analytiques (UMR 5280 - CNRS, ENS Lyon, UCB Lyon 1), Université de Lyon, 5 rue de la Doua, 69100, Villeurbanne, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Centre de RMN à Très Hauts Champs, Institut des Sciences Analytiques (UMR 5280 - CNRS, ENS Lyon, UCB Lyon 1), Université de Lyon, 5 rue de la Doua, 69100, Villeurbanne</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Villeurbanne</settlement></placeName></affiliation></author><author><name sortKey="Jaudzems, Kristaps" sort="Jaudzems, Kristaps" uniqKey="Jaudzems K" first="Kristaps" last="Jaudzems">Kristaps Jaudzems</name><affiliation wicri:level="3"><nlm:affiliation>Centre de RMN à Très Hauts Champs, Institut des Sciences Analytiques (UMR 5280 - CNRS, ENS Lyon, UCB Lyon 1), Université de Lyon, 5 rue de la Doua, 69100, Villeurbanne, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Centre de RMN à Très Hauts Champs, Institut des Sciences Analytiques (UMR 5280 - CNRS, ENS Lyon, UCB Lyon 1), Université de Lyon, 5 rue de la Doua, 69100, Villeurbanne</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Villeurbanne</settlement></placeName></affiliation></author><author><name sortKey="Cala, Diane" sort="Cala, Diane" uniqKey="Cala D" first="Diane" last="Cala">Diane Cala</name><affiliation wicri:level="3"><nlm:affiliation>Centre de RMN à Très Hauts Champs, Institut des Sciences Analytiques (UMR 5280 - CNRS, ENS Lyon, UCB Lyon 1), Université de Lyon, 5 rue de la Doua, 69100, Villeurbanne, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Centre de RMN à Très Hauts Champs, Institut des Sciences Analytiques (UMR 5280 - CNRS, ENS Lyon, UCB Lyon 1), Université de Lyon, 5 rue de la Doua, 69100, Villeurbanne</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Villeurbanne</settlement></placeName></affiliation></author><author><name sortKey="Lalli, Daniela" sort="Lalli, Daniela" uniqKey="Lalli D" first="Daniela" last="Lalli">Daniela Lalli</name><affiliation wicri:level="3"><nlm:affiliation>Centre de RMN à Très Hauts Champs, Institut des Sciences Analytiques (UMR 5280 - CNRS, ENS Lyon, UCB Lyon 1), Université de Lyon, 5 rue de la Doua, 69100, Villeurbanne, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Centre de RMN à Très Hauts Champs, Institut des Sciences Analytiques (UMR 5280 - CNRS, ENS Lyon, UCB Lyon 1), Université de Lyon, 5 rue de la Doua, 69100, Villeurbanne</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Villeurbanne</settlement></placeName></affiliation></author><author><name sortKey="Bertarello, Andrea" sort="Bertarello, Andrea" uniqKey="Bertarello A" first="Andrea" last="Bertarello">Andrea Bertarello</name><affiliation wicri:level="3"><nlm:affiliation>Centre de RMN à Très Hauts Champs, Institut des Sciences Analytiques (UMR 5280 - CNRS, ENS Lyon, UCB Lyon 1), Université de Lyon, 5 rue de la Doua, 69100, Villeurbanne, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Centre de RMN à Très Hauts Champs, Institut des Sciences Analytiques (UMR 5280 - CNRS, ENS Lyon, UCB Lyon 1), Université de Lyon, 5 rue de la Doua, 69100, Villeurbanne</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Villeurbanne</settlement></placeName></affiliation></author><author><name sortKey="Schubeis, Tobias" sort="Schubeis, Tobias" uniqKey="Schubeis T" first="Tobias" last="Schubeis">Tobias Schubeis</name><affiliation wicri:level="3"><nlm:affiliation>Centre de RMN à Très Hauts Champs, Institut des Sciences Analytiques (UMR 5280 - CNRS, ENS Lyon, UCB Lyon 1), Université de Lyon, 5 rue de la Doua, 69100, Villeurbanne, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Centre de RMN à Très Hauts Champs, Institut des Sciences Analytiques (UMR 5280 - CNRS, ENS Lyon, UCB Lyon 1), Université de Lyon, 5 rue de la Doua, 69100, Villeurbanne</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Villeurbanne</settlement></placeName></affiliation></author><author><name sortKey="Akopjana, Inara" sort="Akopjana, Inara" uniqKey="Akopjana I" first="Inara" last="Akopjana">Inara Akopjana</name><affiliation wicri:level="1"><nlm:affiliation>Biomedical Research and Study Centre, Rātsupītes 1, LV1067, Riga, Latvia.</nlm:affiliation><country xml:lang="fr">Lettonie</country><wicri:regionArea>Biomedical Research and Study Centre, Rātsupītes 1, LV1067, Riga</wicri:regionArea><wicri:noRegion>Riga</wicri:noRegion></affiliation></author><author><name sortKey="Kotelovica, Svetlana" sort="Kotelovica, Svetlana" uniqKey="Kotelovica S" first="Svetlana" last="Kotelovica">Svetlana Kotelovica</name><affiliation wicri:level="1"><nlm:affiliation>Biomedical Research and Study Centre, Rātsupītes 1, LV1067, Riga, Latvia.</nlm:affiliation><country xml:lang="fr">Lettonie</country><wicri:regionArea>Biomedical Research and Study Centre, Rātsupītes 1, LV1067, Riga</wicri:regionArea><wicri:noRegion>Riga</wicri:noRegion></affiliation></author><author><name sortKey="Tars, Kaspars" sort="Tars, Kaspars" uniqKey="Tars K" first="Kaspars" last="Tars">Kaspars Tars</name><affiliation wicri:level="1"><nlm:affiliation>Biomedical Research and Study Centre, Rātsupītes 1, LV1067, Riga, Latvia.</nlm:affiliation><country xml:lang="fr">Lettonie</country><wicri:regionArea>Biomedical Research and Study Centre, Rātsupītes 1, LV1067, Riga</wicri:regionArea><wicri:noRegion>Riga</wicri:noRegion></affiliation></author><author><name sortKey="Pica, Andrea" sort="Pica, Andrea" uniqKey="Pica A" first="Andrea" last="Pica">Andrea Pica</name><affiliation wicri:level="1"><nlm:affiliation>Department of Chemical Sciences, University of Naples Federico II, Via Cintia, 80126, Naples, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Chemical Sciences, University of Naples Federico II, Via Cintia, 80126, Naples</wicri:regionArea><wicri:noRegion>Naples</wicri:noRegion></affiliation></author><author><name sortKey="Leone, Serena" sort="Leone, Serena" uniqKey="Leone S" first="Serena" last="Leone">Serena Leone</name><affiliation wicri:level="1"><nlm:affiliation>Department of Chemical Sciences, University of Naples Federico II, Via Cintia, 80126, Naples, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Chemical Sciences, University of Naples Federico II, Via Cintia, 80126, Naples</wicri:regionArea><wicri:noRegion>Naples</wicri:noRegion></affiliation></author><author><name sortKey="Picone, Delia" sort="Picone, Delia" uniqKey="Picone D" first="Delia" last="Picone">Delia Picone</name><affiliation wicri:level="1"><nlm:affiliation>Department of Chemical Sciences, University of Naples Federico II, Via Cintia, 80126, Naples, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Chemical Sciences, University of Naples Federico II, Via Cintia, 80126, Naples</wicri:regionArea><wicri:noRegion>Naples</wicri:noRegion></affiliation></author><author><name sortKey="Xu, Zhi Qiang" sort="Xu, Zhi Qiang" uniqKey="Xu Z" first="Zhi-Qiang" last="Xu">Zhi-Qiang Xu</name><affiliation wicri:level="1"><nlm:affiliation>School of Chemistry, University of Wollongong, NSW, 2522, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>School of Chemistry, University of Wollongong, NSW, 2522</wicri:regionArea><wicri:noRegion>2522</wicri:noRegion></affiliation></author><author><name sortKey="Dixon, Nicholas E" sort="Dixon, Nicholas E" uniqKey="Dixon N" first="Nicholas E" last="Dixon">Nicholas E. Dixon</name><affiliation wicri:level="1"><nlm:affiliation>School of Chemistry, University of Wollongong, NSW, 2522, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>School of Chemistry, University of Wollongong, NSW, 2522</wicri:regionArea><wicri:noRegion>2522</wicri:noRegion></affiliation></author><author><name sortKey="Martinez, Denis" sort="Martinez, Denis" uniqKey="Martinez D" first="Denis" last="Martinez">Denis Martinez</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Chemistry & Biology of Membranes & Nanoobjects (UMR 5248 CBMN - CNRS, University of Bordeaux, Bordeaux INP), All. Geoffroy Saint-Hillaire, 33600, Pessac, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Institute of Chemistry & Biology of Membranes & Nanoobjects (UMR 5248 CBMN - CNRS, University of Bordeaux, Bordeaux INP), All. Geoffroy Saint-Hillaire, 33600, Pessac</wicri:regionArea><wicri:noRegion>33600, Pessac</wicri:noRegion><wicri:noRegion>33600, Pessac</wicri:noRegion></affiliation></author><author><name sortKey="Berbon, Melanie" sort="Berbon, Melanie" uniqKey="Berbon M" first="Mélanie" last="Berbon">Mélanie Berbon</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Chemistry & Biology of Membranes & Nanoobjects (UMR 5248 CBMN - CNRS, University of Bordeaux, Bordeaux INP), All. Geoffroy Saint-Hillaire, 33600, Pessac, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Institute of Chemistry & Biology of Membranes & Nanoobjects (UMR 5248 CBMN - CNRS, University of Bordeaux, Bordeaux INP), All. Geoffroy Saint-Hillaire, 33600, Pessac</wicri:regionArea><wicri:noRegion>33600, Pessac</wicri:noRegion><wicri:noRegion>33600, Pessac</wicri:noRegion></affiliation></author><author><name sortKey="El Mammeri, Nadia" sort="El Mammeri, Nadia" uniqKey="El Mammeri N" first="Nadia" last="El Mammeri">Nadia El Mammeri</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Chemistry & Biology of Membranes & Nanoobjects (UMR 5248 CBMN - CNRS, University of Bordeaux, Bordeaux INP), All. Geoffroy Saint-Hillaire, 33600, Pessac, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Institute of Chemistry & Biology of Membranes & Nanoobjects (UMR 5248 CBMN - CNRS, University of Bordeaux, Bordeaux INP), All. Geoffroy Saint-Hillaire, 33600, Pessac</wicri:regionArea><wicri:noRegion>33600, Pessac</wicri:noRegion><wicri:noRegion>33600, Pessac</wicri:noRegion></affiliation></author><author><name sortKey="Noubhani, Abdelmajid" sort="Noubhani, Abdelmajid" uniqKey="Noubhani A" first="Abdelmajid" last="Noubhani">Abdelmajid Noubhani</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Chemistry & Biology of Membranes & Nanoobjects (UMR 5248 CBMN - CNRS, University of Bordeaux, Bordeaux INP), All. Geoffroy Saint-Hillaire, 33600, Pessac, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Institute of Chemistry & Biology of Membranes & Nanoobjects (UMR 5248 CBMN - CNRS, University of Bordeaux, Bordeaux INP), All. Geoffroy Saint-Hillaire, 33600, Pessac</wicri:regionArea><wicri:noRegion>33600, Pessac</wicri:noRegion><wicri:noRegion>33600, Pessac</wicri:noRegion></affiliation></author><author><name sortKey="Saupe, Sven" sort="Saupe, Sven" uniqKey="Saupe S" first="Sven" last="Saupe">Sven Saupe</name><affiliation wicri:level="4"><nlm:affiliation>Institut de Biochimie et de Génétique Cellulaire (UMR 5095, CNRS -, Université de Bordeaux), 33077, Bordeaux, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Institut de Biochimie et de Génétique Cellulaire (UMR 5095, CNRS -, Université de Bordeaux), 33077, Bordeaux</wicri:regionArea><wicri:noRegion>33077, Bordeaux</wicri:noRegion><orgName type="university">Université de Bordeaux</orgName><placeName><settlement type="city">Bordeaux</settlement><region type="region" nuts="2">Nouvelle-Aquitaine</region><region type="old region" nuts="2">Aquitaine</region></placeName></affiliation></author><author><name sortKey="Habenstein, Birgit" sort="Habenstein, Birgit" uniqKey="Habenstein B" first="Birgit" last="Habenstein">Birgit Habenstein</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Chemistry & Biology of Membranes & Nanoobjects (UMR 5248 CBMN - CNRS, University of Bordeaux, Bordeaux INP), All. Geoffroy Saint-Hillaire, 33600, Pessac, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Institute of Chemistry & Biology of Membranes & Nanoobjects (UMR 5248 CBMN - CNRS, University of Bordeaux, Bordeaux INP), All. Geoffroy Saint-Hillaire, 33600, Pessac</wicri:regionArea><wicri:noRegion>33600, Pessac</wicri:noRegion><wicri:noRegion>33600, Pessac</wicri:noRegion></affiliation></author><author><name sortKey="Loquet, Antoine" sort="Loquet, Antoine" uniqKey="Loquet A" first="Antoine" last="Loquet">Antoine Loquet</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Chemistry & Biology of Membranes & Nanoobjects (UMR 5248 CBMN - CNRS, University of Bordeaux, Bordeaux INP), All. Geoffroy Saint-Hillaire, 33600, Pessac, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Institute of Chemistry & Biology of Membranes & Nanoobjects (UMR 5248 CBMN - CNRS, University of Bordeaux, Bordeaux INP), All. Geoffroy Saint-Hillaire, 33600, Pessac</wicri:regionArea><wicri:noRegion>33600, Pessac</wicri:noRegion><wicri:noRegion>33600, Pessac</wicri:noRegion></affiliation></author><author><name sortKey="Pintacuda, Guido" sort="Pintacuda, Guido" uniqKey="Pintacuda G" first="Guido" last="Pintacuda">Guido Pintacuda</name><affiliation wicri:level="1"><nlm:affiliation>Centre de RMN à Très Hauts Champs, Institut des Sciences Analytiques (UMR 5280 - CNRS, ENS Lyon, UCB Lyon 1), Université de Lyon, 5 rue de la Doua, 69100, Villeurbanne, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Centre de RMN à Très Hauts Champs, Institut des Sciences Analytiques (UMR 5280 - CNRS, ENS Lyon, UCB Lyon 1), Université de Lyon, 5 rue de la Doua, 69100, Villeurbanne</wicri:regionArea><wicri:noRegion>69100, Villeurbanne</wicri:noRegion><wicri:noRegion>69100, Villeurbanne</wicri:noRegion></affiliation></author></analytic><series><title level="j">Angewandte Chemie (International ed. in English)</title><idno type="eISSN">1521-3773</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We demonstrate sensitive detection of alpha protons of fully protonated proteins by solid-state NMR spectroscopy with 100-111 kHz magic-angle spinning (MAS). The excellent resolution in the Cα-Hα plane is demonstrated for 5 proteins, including microcrystals, a sedimented complex, a capsid and amyloid fibrils. A set of 3D spectra based on a Cα-Hα detection block was developed and applied for the sequence-specific backbone and aliphatic side-chain resonance assignment using only 500 μg of sample. These developments accelerate structural studies of biomolecular assemblies available in submilligram quantities without the need of protein deuteration.</div></front></TEI><affiliations><list><country><li>Australie</li><li>France</li><li>Italie</li><li>Lettonie</li></country><region><li>Aquitaine</li><li>Auvergne-Rhône-Alpes</li><li>Nouvelle-Aquitaine</li><li>Rhône-Alpes</li></region><settlement><li>Bordeaux</li><li>Villeurbanne</li></settlement><orgName><li>Université de Bordeaux</li></orgName></list><tree><country name="France"><region name="Auvergne-Rhône-Alpes"><name sortKey="Stanek, Jan" sort="Stanek, Jan" uniqKey="Stanek J" first="Jan" last="Stanek">Jan Stanek</name></region><name sortKey="Andreas, Loren B" sort="Andreas, Loren B" uniqKey="Andreas L" first="Loren B" last="Andreas">Loren B. Andreas</name><name sortKey="Berbon, Melanie" sort="Berbon, Melanie" uniqKey="Berbon M" first="Mélanie" last="Berbon">Mélanie Berbon</name><name sortKey="Bertarello, Andrea" sort="Bertarello, Andrea" uniqKey="Bertarello A" first="Andrea" last="Bertarello">Andrea Bertarello</name><name sortKey="Cala, Diane" sort="Cala, Diane" uniqKey="Cala D" first="Diane" last="Cala">Diane Cala</name><name sortKey="El Mammeri, Nadia" sort="El Mammeri, Nadia" uniqKey="El Mammeri N" first="Nadia" last="El Mammeri">Nadia El Mammeri</name><name sortKey="Habenstein, Birgit" sort="Habenstein, Birgit" uniqKey="Habenstein B" first="Birgit" last="Habenstein">Birgit Habenstein</name><name sortKey="Jaudzems, Kristaps" sort="Jaudzems, Kristaps" uniqKey="Jaudzems K" first="Kristaps" last="Jaudzems">Kristaps Jaudzems</name><name sortKey="Lalli, Daniela" sort="Lalli, Daniela" uniqKey="Lalli D" first="Daniela" last="Lalli">Daniela Lalli</name><name sortKey="Loquet, Antoine" sort="Loquet, Antoine" uniqKey="Loquet A" first="Antoine" last="Loquet">Antoine Loquet</name><name sortKey="Martinez, Denis" sort="Martinez, Denis" uniqKey="Martinez D" first="Denis" last="Martinez">Denis Martinez</name><name sortKey="Noubhani, Abdelmajid" sort="Noubhani, Abdelmajid" uniqKey="Noubhani A" first="Abdelmajid" last="Noubhani">Abdelmajid Noubhani</name><name sortKey="Pintacuda, Guido" sort="Pintacuda, Guido" uniqKey="Pintacuda G" first="Guido" last="Pintacuda">Guido Pintacuda</name><name sortKey="Saupe, Sven" sort="Saupe, Sven" uniqKey="Saupe S" first="Sven" last="Saupe">Sven Saupe</name><name sortKey="Schubeis, Tobias" sort="Schubeis, Tobias" uniqKey="Schubeis T" first="Tobias" last="Schubeis">Tobias Schubeis</name></country><country name="Lettonie"><noRegion><name sortKey="Akopjana, Inara" sort="Akopjana, Inara" uniqKey="Akopjana I" first="Inara" last="Akopjana">Inara Akopjana</name></noRegion><name sortKey="Kotelovica, Svetlana" sort="Kotelovica, Svetlana" uniqKey="Kotelovica S" first="Svetlana" last="Kotelovica">Svetlana Kotelovica</name><name sortKey="Tars, Kaspars" sort="Tars, Kaspars" uniqKey="Tars K" first="Kaspars" last="Tars">Kaspars Tars</name></country><country name="Italie"><noRegion><name sortKey="Pica, Andrea" sort="Pica, Andrea" uniqKey="Pica A" first="Andrea" last="Pica">Andrea Pica</name></noRegion><name sortKey="Leone, Serena" sort="Leone, Serena" uniqKey="Leone S" first="Serena" last="Leone">Serena Leone</name><name sortKey="Picone, Delia" sort="Picone, Delia" uniqKey="Picone D" first="Delia" last="Picone">Delia Picone</name></country><country name="Australie"><noRegion><name sortKey="Xu, Zhi Qiang" sort="Xu, Zhi Qiang" uniqKey="Xu Z" first="Zhi-Qiang" last="Xu">Zhi-Qiang Xu</name></noRegion><name sortKey="Dixon, Nicholas E" sort="Dixon, Nicholas E" uniqKey="Dixon N" first="Nicholas E" last="Dixon">Nicholas E. Dixon</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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